Natural transformation of Thermotoga sp. strain RQ7

Background Thermotoga species are organisms of enormous interest from a biotechnological as well as evolutionary point of view. Genetic modifications of Thermotoga spp. are often desired in order to fully release their multifarious potentials. Effective transformation of recombinant DNA into these bacteria constitutes a critical step of such efforts. This study aims to establish natural competency in Thermotoga spp. and to provide a convenient method to transform these organisms. Results Foreign DNA was found to be relatively stable in the supernatant of a Thermotoga culture for up to 6 hours. Adding donor DNA to T. sp. strain RQ7 at its early exponential growth phase (OD600 0.18 ~ 0.20) resulted in direct acquisition of the DNA by the cells. Both T. neapolitana chromosomal DNA and Thermotoga-E. coli shuttle vectors effectively transformed T. sp. strain RQ7, rendering the cells resistance to kanamycin. The kan gene carried by the shuttle vector pDH10 was detected by PCR from the plasmid extract of the transformants, and the amplicons were verified by restriction digestions. A procedure for natural transformation of Thermotoga spp. was established and optimized. With the optimized method, T. sp. strain RQ7 sustained a transformation frequency in the order of 10−7 with both genomic and plasmid DNA

(E)-N′-(2,5-Dimethoxy­benzyl­idene)-3,4-dihydroxy­benzohydrazide monohydrate

In the title compound, C16H16N2O5·H2O, the dihedral angle between the two benzene rings is 25.9 (1)°. Intra­molecular O—H⋯O and N—H⋯O hydrogen bonds are observed. In the crystal, the components are linked into a three-dimensional network by O—H⋯O and O—H⋯(O,O) hydrogen bonds

Ghost imaging lidar via sparsity constraints

For remote sensing, high-resolution imaging techniques are helpful to catch more characteristic information of the target. We extend pseudo-thermal light ghost imaging to the area of remote imaging and propose a ghost imaging lidar system. For the first time, we demonstrate experimentally that the real-space image of a target at about 1.0 km range with 20 mm resolution is achieved by ghost imaging via sparsity constraints (GISC) technique. The characters of GISC technique compared to the existing lidar systems are also discussed.Comment: 4pages, 3figure